Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii
نویسندگان
چکیده
منابع مشابه
Succinic dehydrogenase of Azotobacter vinelandii.
During the investigation of the Krebs cycle in Azotobacter agile, strain 4.4 (Repaske and Wilson, 1953), it was found that whole cells normally required 20 minutes before adaptation to succinate, but succinoxidase activity could be demonstrated in cell-free extracts made from unadapted cells. If extracts prepared in a 10 kc Raytheon sonic oscillator were centrifuged at 145,000 G for 30 minutes,...
متن کاملThe GacS sensor kinase regulates alginate and poly-beta-hydroxybutyrate production in Azotobacter vinelandii.
Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and the intracellular polyester poly-beta-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from an A. vinelandii gene library diminished alginate production by A. vinelandii mucoid strain ATCC 9046. The nucleotide sequence and predicted amino acid sequence of the locus responsible for the mucoidy suppression ...
متن کاملThe global regulators GacA and sigma(S) form part of a cascade that controls alginate production in Azotobacter vinelandii.
Transcription of the Azotobacter vinelandii algD gene, which encodes GDP-mannose dehydrogenase (the rate-limiting enzyme of alginate synthesis), starts from three sites: p1, p2, and p3. The sensor kinase GacS, a member of the two-component regulatory system, is required for transcription of algD from its three sites during the stationary phase. Here we show that algD is expressed constitutively...
متن کاملThe Global Regulators GacA and S Form Part of a Cascade That Controls Alginate Production in Azotobacter vinelandii
Transcription of the Azotobacter vinelandii algD gene, which encodes GDP-mannose dehydrogenase (the rate-limiting enzyme of alginate synthesis), starts from three sites: p1, p2, and p3. The sensor kinase GacS, a member of the two-component regulatory system, is required for transcription of algD from its three sites during the stationary phase. Here we show that algD is expressed constitutively...
متن کاملRole of alternative sigma factor algU in encystment of Azotobacter vinelandii.
Alginate is essential for encystment in Azotobacter vinelandii. Transcription of the algD gene, which codes for GDP-mannose dehydrogenase, a key enzyme in the alginate biosynthetic pathway, is initiated at two promoters, one of which, p2, has sigmaE consensus sequences. AlgU is the A. vinelandii alternative sigmaE factor. In this study, we constructed an algU mutant (SMU88) which, as expected, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1996
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.178.7.1793-1799.1996